Removal of Inhibitory Effects in a Serum Cardiac Troponin I Immunoassay

Removal of inhibitory effects in a serum cardiac troponin I immunoassay.

The measurement of cardiac troponin I (cTnI) has become the gold standard for the clinical diagnosis of myocardial infarction (1 ). Varieties of commercial sandwich-type immunoassays are used for the measurement of cTnI concentration in human serum or plasma. Several of these assays use pairs of monoclonal antihuman cTnI antibodies with high specificities for the invariant part of the cTnI mole...

Degradation of cardiac troponin I in serum complicates comparisons of cardiac troponin I assays.

BACKGROUND Up to a 20-fold variation in serum cardiac troponin I (cTnI) concentration may be observed for a given patient sample with different analytical methods. Because more limited variation is seen for control materials and for purified cTnI, we explored the possibility that cTnI was present in altered forms in serum. METHODS We used four recombinantly engineered cTnI fragments to study ...

Chimeric recombinant antibody fragments in cardiac troponin I immunoassay.

OBJECTIVES To introduce a novel nanoparticle-based immunoassay for cardiac troponin I (cTnI) utilizing chimeric antibody fragments and to demonstrate that removal of antibody Fc-part and antibody chimerization decrease matrix related interferences. DESIGN AND METHODS A sandwich-type immunoassay for cTnI based on recombinant chimeric (mouse variable/human constant) antigen binding (cFab) antib...

False-negative immunoassay results for cardiac troponin I probably due to circulating troponin I autoantibodies.

Differential reactivity of cardiac and skeletal muscle from various species in a cardiac troponin I immunoassay.

To identify a blood test that can differentiate cardiac from skeletal muscle injury in animals, we compared tissue reactivities for various species with the use of an immunoassay for human cardiac troponin I (cTnI). Tissue reactivity varied as a function of the homology of tissue troponin with human cTnI. Cardiac reactivity in large mammals was equivalent to cTnI, 9.8 +/- 0.6 mg/g, and was 2-fo...